spike neutralizing antibody Search Results


spike neutralizing antibody  (Sino Biological)
95
Sino Biological spike neutralizing antibody
(A-B) A549:EV and A549:N cells were infected with WT (MOI 1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for confocal microscopy. (A-B) Maximum intensity projections of merged dsRNA ( A-B , red) staining with the N protein ( A , white) or Nsp6 ( B , white) in A549-EV and A549:N cells. Nuclei were Hoechst stained (blue). Note that DVG-B encoded GFP is depicted (green). Scale bar indicates 20μm. Insets depict magnified cell regions indicated by the white boxes of dsRNA and N protein co-staining ( A ) or dsRNA and Nsp6 co-staining ( B ). Scale bar indicates 5μm. Graphs indicate the Pearson’s correlation coefficient ( R) values for the co-localization of dsRNA and N ( C, above) or dsRNA and Nsp6 ( C, below ) in A549:EV and A549:N cells, respectively. Individual values are shown. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by one-way ANOVA with Turkey’s multiple comparisons test. Mean±SD. ( D ) A549:EV (left) and A549:N cells (right) were infected with WT (MOI 1) or DVG-B (MOI 50 to maximize the DVG-B infected cell number), respectively, and cells were collected 24-hours post-inoculation for fixation and processing for transmission electron microscopy. Scale bars indicate 2μm. Insets depict magnified cell regions indicated by the white boxes. Scale bars indicate 500nm. Black asterisks indicate single membrane vesicles, red asterisks indicate lysosome-like structures. ( E ) Due to significant more cell death in WT infected A549:EV and A549:N cells, we reduced MOI of WT virus to 0.1 for this experiment. A549:EV and A549:N cells were infected with WT (MOI 0.1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IFNB1, IL-29 and ISG54 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. Because the A549:N cell line expresses codon-optimized N, the qPCR primers specifically detect virus-derived N transcripts. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=2 biological repeats, mean±SD. ( F ) A549:EV and A549:N cells were inoculated as in ( E ). Immediately following 2-hours of inoculation, 10μg/mL of a <t>neutralizing</t> SARS-CoV-2 spike antibody or 10μg/mL of an IgG isotype control antibody were added to the infected cell cultures. 24-hours post inoculation cell supernatants were collected for virus titer determination by TCID 50 /mL quantification. Cells were collected for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IL-29 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=3 biological repeats, mean±SD.
Spike Neutralizing Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spike neutralizing antibody/product/Sino Biological
Average 95 stars, based on 1 article reviews
spike neutralizing antibody - by Bioz Stars, 2026-06
95/100 stars
  Buy from Supplier
anti sars cov 2 neutralizing antibody nab human igg1 sad535  (ACROBiosystems)
95
ACROBiosystems anti sars cov 2 neutralizing antibody nab human igg1 sad535
(A-B) A549:EV and A549:N cells were infected with WT (MOI 1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for confocal microscopy. (A-B) Maximum intensity projections of merged dsRNA ( A-B , red) staining with the N protein ( A , white) or Nsp6 ( B , white) in A549-EV and A549:N cells. Nuclei were Hoechst stained (blue). Note that DVG-B encoded GFP is depicted (green). Scale bar indicates 20μm. Insets depict magnified cell regions indicated by the white boxes of dsRNA and N protein co-staining ( A ) or dsRNA and Nsp6 co-staining ( B ). Scale bar indicates 5μm. Graphs indicate the Pearson’s correlation coefficient ( R) values for the co-localization of dsRNA and N ( C, above) or dsRNA and Nsp6 ( C, below ) in A549:EV and A549:N cells, respectively. Individual values are shown. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by one-way ANOVA with Turkey’s multiple comparisons test. Mean±SD. ( D ) A549:EV (left) and A549:N cells (right) were infected with WT (MOI 1) or DVG-B (MOI 50 to maximize the DVG-B infected cell number), respectively, and cells were collected 24-hours post-inoculation for fixation and processing for transmission electron microscopy. Scale bars indicate 2μm. Insets depict magnified cell regions indicated by the white boxes. Scale bars indicate 500nm. Black asterisks indicate single membrane vesicles, red asterisks indicate lysosome-like structures. ( E ) Due to significant more cell death in WT infected A549:EV and A549:N cells, we reduced MOI of WT virus to 0.1 for this experiment. A549:EV and A549:N cells were infected with WT (MOI 0.1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IFNB1, IL-29 and ISG54 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. Because the A549:N cell line expresses codon-optimized N, the qPCR primers specifically detect virus-derived N transcripts. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=2 biological repeats, mean±SD. ( F ) A549:EV and A549:N cells were inoculated as in ( E ). Immediately following 2-hours of inoculation, 10μg/mL of a <t>neutralizing</t> SARS-CoV-2 spike antibody or 10μg/mL of an IgG isotype control antibody were added to the infected cell cultures. 24-hours post inoculation cell supernatants were collected for virus titer determination by TCID 50 /mL quantification. Cells were collected for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IL-29 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=3 biological repeats, mean±SD.
Anti Sars Cov 2 Neutralizing Antibody Nab Human Igg1 Sad535, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti sars cov 2 neutralizing antibody nab human igg1 sad535/product/ACROBiosystems
Average 95 stars, based on 1 article reviews
anti sars cov 2 neutralizing antibody nab human igg1 sad535 - by Bioz Stars, 2026-06
95/100 stars
  Buy from Supplier
anti sars cov 2 s1 neutralizing antibody mouse mab ab1  (Sino Biological)
95
Sino Biological anti sars cov 2 s1 neutralizing antibody mouse mab ab1
(A-B) A549:EV and A549:N cells were infected with WT (MOI 1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for confocal microscopy. (A-B) Maximum intensity projections of merged dsRNA ( A-B , red) staining with the N protein ( A , white) or Nsp6 ( B , white) in A549-EV and A549:N cells. Nuclei were Hoechst stained (blue). Note that DVG-B encoded GFP is depicted (green). Scale bar indicates 20μm. Insets depict magnified cell regions indicated by the white boxes of dsRNA and N protein co-staining ( A ) or dsRNA and Nsp6 co-staining ( B ). Scale bar indicates 5μm. Graphs indicate the Pearson’s correlation coefficient ( R) values for the co-localization of dsRNA and N ( C, above) or dsRNA and Nsp6 ( C, below ) in A549:EV and A549:N cells, respectively. Individual values are shown. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by one-way ANOVA with Turkey’s multiple comparisons test. Mean±SD. ( D ) A549:EV (left) and A549:N cells (right) were infected with WT (MOI 1) or DVG-B (MOI 50 to maximize the DVG-B infected cell number), respectively, and cells were collected 24-hours post-inoculation for fixation and processing for transmission electron microscopy. Scale bars indicate 2μm. Insets depict magnified cell regions indicated by the white boxes. Scale bars indicate 500nm. Black asterisks indicate single membrane vesicles, red asterisks indicate lysosome-like structures. ( E ) Due to significant more cell death in WT infected A549:EV and A549:N cells, we reduced MOI of WT virus to 0.1 for this experiment. A549:EV and A549:N cells were infected with WT (MOI 0.1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IFNB1, IL-29 and ISG54 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. Because the A549:N cell line expresses codon-optimized N, the qPCR primers specifically detect virus-derived N transcripts. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=2 biological repeats, mean±SD. ( F ) A549:EV and A549:N cells were inoculated as in ( E ). Immediately following 2-hours of inoculation, 10μg/mL of a <t>neutralizing</t> SARS-CoV-2 spike antibody or 10μg/mL of an IgG isotype control antibody were added to the infected cell cultures. 24-hours post inoculation cell supernatants were collected for virus titer determination by TCID 50 /mL quantification. Cells were collected for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IL-29 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=3 biological repeats, mean±SD.
Anti Sars Cov 2 S1 Neutralizing Antibody Mouse Mab Ab1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti sars cov 2 s1 neutralizing antibody mouse mab ab1/product/Sino Biological
Average 95 stars, based on 1 article reviews
anti sars cov 2 s1 neutralizing antibody mouse mab ab1 - by Bioz Stars, 2026-06
95/100 stars
  Buy from Supplier
40592 r118  (Sino Biological)
93
Sino Biological 40592 r118
(A-B) A549:EV and A549:N cells were infected with WT (MOI 1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for confocal microscopy. (A-B) Maximum intensity projections of merged dsRNA ( A-B , red) staining with the N protein ( A , white) or Nsp6 ( B , white) in A549-EV and A549:N cells. Nuclei were Hoechst stained (blue). Note that DVG-B encoded GFP is depicted (green). Scale bar indicates 20μm. Insets depict magnified cell regions indicated by the white boxes of dsRNA and N protein co-staining ( A ) or dsRNA and Nsp6 co-staining ( B ). Scale bar indicates 5μm. Graphs indicate the Pearson’s correlation coefficient ( R) values for the co-localization of dsRNA and N ( C, above) or dsRNA and Nsp6 ( C, below ) in A549:EV and A549:N cells, respectively. Individual values are shown. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by one-way ANOVA with Turkey’s multiple comparisons test. Mean±SD. ( D ) A549:EV (left) and A549:N cells (right) were infected with WT (MOI 1) or DVG-B (MOI 50 to maximize the DVG-B infected cell number), respectively, and cells were collected 24-hours post-inoculation for fixation and processing for transmission electron microscopy. Scale bars indicate 2μm. Insets depict magnified cell regions indicated by the white boxes. Scale bars indicate 500nm. Black asterisks indicate single membrane vesicles, red asterisks indicate lysosome-like structures. ( E ) Due to significant more cell death in WT infected A549:EV and A549:N cells, we reduced MOI of WT virus to 0.1 for this experiment. A549:EV and A549:N cells were infected with WT (MOI 0.1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IFNB1, IL-29 and ISG54 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. Because the A549:N cell line expresses codon-optimized N, the qPCR primers specifically detect virus-derived N transcripts. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=2 biological repeats, mean±SD. ( F ) A549:EV and A549:N cells were inoculated as in ( E ). Immediately following 2-hours of inoculation, 10μg/mL of a <t>neutralizing</t> SARS-CoV-2 spike antibody or 10μg/mL of an IgG isotype control antibody were added to the infected cell cultures. 24-hours post inoculation cell supernatants were collected for virus titer determination by TCID 50 /mL quantification. Cells were collected for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IL-29 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=3 biological repeats, mean±SD.
40592 R118, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/40592 r118/product/Sino Biological
Average 93 stars, based on 1 article reviews
40592 r118 - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
monoclonal antibody  (Sino Biological)
93
Sino Biological monoclonal antibody
(A-B) A549:EV and A549:N cells were infected with WT (MOI 1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for confocal microscopy. (A-B) Maximum intensity projections of merged dsRNA ( A-B , red) staining with the N protein ( A , white) or Nsp6 ( B , white) in A549-EV and A549:N cells. Nuclei were Hoechst stained (blue). Note that DVG-B encoded GFP is depicted (green). Scale bar indicates 20μm. Insets depict magnified cell regions indicated by the white boxes of dsRNA and N protein co-staining ( A ) or dsRNA and Nsp6 co-staining ( B ). Scale bar indicates 5μm. Graphs indicate the Pearson’s correlation coefficient ( R) values for the co-localization of dsRNA and N ( C, above) or dsRNA and Nsp6 ( C, below ) in A549:EV and A549:N cells, respectively. Individual values are shown. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by one-way ANOVA with Turkey’s multiple comparisons test. Mean±SD. ( D ) A549:EV (left) and A549:N cells (right) were infected with WT (MOI 1) or DVG-B (MOI 50 to maximize the DVG-B infected cell number), respectively, and cells were collected 24-hours post-inoculation for fixation and processing for transmission electron microscopy. Scale bars indicate 2μm. Insets depict magnified cell regions indicated by the white boxes. Scale bars indicate 500nm. Black asterisks indicate single membrane vesicles, red asterisks indicate lysosome-like structures. ( E ) Due to significant more cell death in WT infected A549:EV and A549:N cells, we reduced MOI of WT virus to 0.1 for this experiment. A549:EV and A549:N cells were infected with WT (MOI 0.1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IFNB1, IL-29 and ISG54 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. Because the A549:N cell line expresses codon-optimized N, the qPCR primers specifically detect virus-derived N transcripts. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=2 biological repeats, mean±SD. ( F ) A549:EV and A549:N cells were inoculated as in ( E ). Immediately following 2-hours of inoculation, 10μg/mL of a <t>neutralizing</t> SARS-CoV-2 spike antibody or 10μg/mL of an IgG isotype control antibody were added to the infected cell cultures. 24-hours post inoculation cell supernatants were collected for virus titer determination by TCID 50 /mL quantification. Cells were collected for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IL-29 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=3 biological repeats, mean±SD.
Monoclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal antibody/product/Sino Biological
Average 93 stars, based on 1 article reviews
monoclonal antibody - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
sars cov  (Sino Biological)
94
Sino Biological sars cov
Comparison of the <t>SARS-CoV-2</t> polyclonal original-D614G, passage 60 and 71, and the recombinant P70rec viruses. ( a – c ) VeroCCL81 cells were infected at a multiplicity of infection of 0.001 with polyclonal passage (P) 4 (original-D614G virus), P60, or P71 virus stocks or the recombinant virus P70rec (n = 1 culture per virus), supernatant was collected at day 1–6 post-infection, and cultures were evaluated for ( a ) supernatant infectious titers (50% tissue culture infectious dose (TCID 50 )/mL) with dots representing geometric means of four technical replicates with standard deviations, ( b ) supernatant virus genome titers (RNA copies/mL), and ( c ) cytopathic effect evaluated by microscope inspection. ( d ) Original-D614G, P60, and P70rec viruses as specified above the graphs were produced in a fixed-bed bioreactor. Infectious titers were determined for the virus-containing medium collected upon each medium-replacement, bars represent geometric means of two to three technical replicates with standard deviations (left y-axis). The accumulated yield of TCID 50 units obtained from each culture was calculated based on the infectious titers and the volume of collected virus-containing medium (right y-axis). ( e ) 50% inhibitory dilution (ID 50 ) values against P60, P70rec, and P71 viruses for plasma from infection-naïve SARS-CoV-2 vaccinated (n = 6) or vaccination-naïve convalescent (n = 6) individuals. ( f ) 50% inhibitory concentration (IC 50 ) values for neutralization by monoclonal antibody bebtelovimab. ID 50 and IC 50 values were calculated based on curves shown in Supplementary Fig. . For ( e ) the three groups were compared in a Friedman test (line) with a post-hoc Dunn’s multiple comparison test (line with brackets). ** p < 0.005; ns, p > 0.05. LLOQ, lower limit of quantification. #, cytopathic effect not evaluated due to low numbers of cells because of SARS-CoV-2 induced cell death.
Sars Cov, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sars cov/product/Sino Biological
Average 94 stars, based on 1 article reviews
sars cov - by Bioz Stars, 2026-06
94/100 stars
  Buy from Supplier
sars cov 2 2019 ncov spike neutralizing antibody  (Sino Biological)
93
Sino Biological sars cov 2 2019 ncov spike neutralizing antibody
Comparison of the <t>SARS-CoV-2</t> polyclonal original-D614G, passage 60 and 71, and the recombinant P70rec viruses. ( a – c ) VeroCCL81 cells were infected at a multiplicity of infection of 0.001 with polyclonal passage (P) 4 (original-D614G virus), P60, or P71 virus stocks or the recombinant virus P70rec (n = 1 culture per virus), supernatant was collected at day 1–6 post-infection, and cultures were evaluated for ( a ) supernatant infectious titers (50% tissue culture infectious dose (TCID 50 )/mL) with dots representing geometric means of four technical replicates with standard deviations, ( b ) supernatant virus genome titers (RNA copies/mL), and ( c ) cytopathic effect evaluated by microscope inspection. ( d ) Original-D614G, P60, and P70rec viruses as specified above the graphs were produced in a fixed-bed bioreactor. Infectious titers were determined for the virus-containing medium collected upon each medium-replacement, bars represent geometric means of two to three technical replicates with standard deviations (left y-axis). The accumulated yield of TCID 50 units obtained from each culture was calculated based on the infectious titers and the volume of collected virus-containing medium (right y-axis). ( e ) 50% inhibitory dilution (ID 50 ) values against P60, P70rec, and P71 viruses for plasma from infection-naïve SARS-CoV-2 vaccinated (n = 6) or vaccination-naïve convalescent (n = 6) individuals. ( f ) 50% inhibitory concentration (IC 50 ) values for neutralization by monoclonal antibody bebtelovimab. ID 50 and IC 50 values were calculated based on curves shown in Supplementary Fig. . For ( e ) the three groups were compared in a Friedman test (line) with a post-hoc Dunn’s multiple comparison test (line with brackets). ** p < 0.005; ns, p > 0.05. LLOQ, lower limit of quantification. #, cytopathic effect not evaluated due to low numbers of cells because of SARS-CoV-2 induced cell death.
Sars Cov 2 2019 Ncov Spike Neutralizing Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sars cov 2 2019 ncov spike neutralizing antibody/product/Sino Biological
Average 93 stars, based on 1 article reviews
sars cov 2 2019 ncov spike neutralizing antibody - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
sars cov 2 2019 ncov spike antibody  (Sino Biological)
94
Sino Biological sars cov 2 2019 ncov spike antibody
Comparison of the <t>SARS-CoV-2</t> polyclonal original-D614G, passage 60 and 71, and the recombinant P70rec viruses. ( a – c ) VeroCCL81 cells were infected at a multiplicity of infection of 0.001 with polyclonal passage (P) 4 (original-D614G virus), P60, or P71 virus stocks or the recombinant virus P70rec (n = 1 culture per virus), supernatant was collected at day 1–6 post-infection, and cultures were evaluated for ( a ) supernatant infectious titers (50% tissue culture infectious dose (TCID 50 )/mL) with dots representing geometric means of four technical replicates with standard deviations, ( b ) supernatant virus genome titers (RNA copies/mL), and ( c ) cytopathic effect evaluated by microscope inspection. ( d ) Original-D614G, P60, and P70rec viruses as specified above the graphs were produced in a fixed-bed bioreactor. Infectious titers were determined for the virus-containing medium collected upon each medium-replacement, bars represent geometric means of two to three technical replicates with standard deviations (left y-axis). The accumulated yield of TCID 50 units obtained from each culture was calculated based on the infectious titers and the volume of collected virus-containing medium (right y-axis). ( e ) 50% inhibitory dilution (ID 50 ) values against P60, P70rec, and P71 viruses for plasma from infection-naïve SARS-CoV-2 vaccinated (n = 6) or vaccination-naïve convalescent (n = 6) individuals. ( f ) 50% inhibitory concentration (IC 50 ) values for neutralization by monoclonal antibody bebtelovimab. ID 50 and IC 50 values were calculated based on curves shown in Supplementary Fig. . For ( e ) the three groups were compared in a Friedman test (line) with a post-hoc Dunn’s multiple comparison test (line with brackets). ** p < 0.005; ns, p > 0.05. LLOQ, lower limit of quantification. #, cytopathic effect not evaluated due to low numbers of cells because of SARS-CoV-2 induced cell death.
Sars Cov 2 2019 Ncov Spike Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sars cov 2 2019 ncov spike antibody/product/Sino Biological
Average 94 stars, based on 1 article reviews
sars cov 2 2019 ncov spike antibody - by Bioz Stars, 2026-06
94/100 stars
  Buy from Supplier
rbd domain  (ProSci Incorporated)
90
ProSci Incorporated rbd domain
Comparison of the <t>SARS-CoV-2</t> polyclonal original-D614G, passage 60 and 71, and the recombinant P70rec viruses. ( a – c ) VeroCCL81 cells were infected at a multiplicity of infection of 0.001 with polyclonal passage (P) 4 (original-D614G virus), P60, or P71 virus stocks or the recombinant virus P70rec (n = 1 culture per virus), supernatant was collected at day 1–6 post-infection, and cultures were evaluated for ( a ) supernatant infectious titers (50% tissue culture infectious dose (TCID 50 )/mL) with dots representing geometric means of four technical replicates with standard deviations, ( b ) supernatant virus genome titers (RNA copies/mL), and ( c ) cytopathic effect evaluated by microscope inspection. ( d ) Original-D614G, P60, and P70rec viruses as specified above the graphs were produced in a fixed-bed bioreactor. Infectious titers were determined for the virus-containing medium collected upon each medium-replacement, bars represent geometric means of two to three technical replicates with standard deviations (left y-axis). The accumulated yield of TCID 50 units obtained from each culture was calculated based on the infectious titers and the volume of collected virus-containing medium (right y-axis). ( e ) 50% inhibitory dilution (ID 50 ) values against P60, P70rec, and P71 viruses for plasma from infection-naïve SARS-CoV-2 vaccinated (n = 6) or vaccination-naïve convalescent (n = 6) individuals. ( f ) 50% inhibitory concentration (IC 50 ) values for neutralization by monoclonal antibody bebtelovimab. ID 50 and IC 50 values were calculated based on curves shown in Supplementary Fig. . For ( e ) the three groups were compared in a Friedman test (line) with a post-hoc Dunn’s multiple comparison test (line with brackets). ** p < 0.005; ns, p > 0.05. LLOQ, lower limit of quantification. #, cytopathic effect not evaluated due to low numbers of cells because of SARS-CoV-2 induced cell death.
Rbd Domain, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rbd domain/product/ProSci Incorporated
Average 90 stars, based on 1 article reviews
rbd domain - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
mm17t  (Sino Biological)
92
Sino Biological mm17t
(A) All B cells (dots) recovered that had paired heavy/light chain sequencing information and antigen/second antibody reactivity information from the AAB-seq experiment for SARS-COV-2 RBD antibodies (n = 3,712) are shown, with CLR(RBD_UMI) (x axis) and AAB-scores (y axis). Antibodies selected dispersedly for expression and validation (colorized dots) are colored by the LG(ELISA_AUC) scores from lowest (purple) to highest (red). (B) Neutralization assays were performed using pseudotyped viruses displaying the S protein from indicated SARS-CoV-2 WT. The values are means ± SD, n = 3. (C) Antibodies were tested for antibody-dependent cellular phagocytosis activity (ADCP) against SARS-CoV-2 RBD, compared to positive control <t>MM17T.</t> AUC of the trogocytosis score is shown, calculated from data in . Data are represented as means ± SDs, n=3. (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the C3b deposition score is shown, calculated from data in . Data are represented as means ± SDs, n=3.
Mm17t, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mm17t/product/Sino Biological
Average 92 stars, based on 1 article reviews
mm17t - by Bioz Stars, 2026-06
92/100 stars
  Buy from Supplier
rabbit 2019 ncov spike s1 antibody  (Sino Biological)
94
Sino Biological rabbit 2019 ncov spike s1 antibody
(A) All B cells (dots) recovered that had paired heavy/light chain sequencing information and antigen/second antibody reactivity information from the AAB-seq experiment for SARS-COV-2 RBD antibodies (n = 3,712) are shown, with CLR(RBD_UMI) (x axis) and AAB-scores (y axis). Antibodies selected dispersedly for expression and validation (colorized dots) are colored by the LG(ELISA_AUC) scores from lowest (purple) to highest (red). (B) Neutralization assays were performed using pseudotyped viruses displaying the S protein from indicated SARS-CoV-2 WT. The values are means ± SD, n = 3. (C) Antibodies were tested for antibody-dependent cellular phagocytosis activity (ADCP) against SARS-CoV-2 RBD, compared to positive control <t>MM17T.</t> AUC of the trogocytosis score is shown, calculated from data in . Data are represented as means ± SDs, n=3. (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the C3b deposition score is shown, calculated from data in . Data are represented as means ± SDs, n=3.
Rabbit 2019 Ncov Spike S1 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit 2019 ncov spike s1 antibody/product/Sino Biological
Average 94 stars, based on 1 article reviews
rabbit 2019 ncov spike s1 antibody - by Bioz Stars, 2026-06
94/100 stars
  Buy from Supplier
sars cov 2 spike neutralizing antibody  (Sino Biological)
93
Sino Biological sars cov 2 spike neutralizing antibody
(A) All B cells (dots) recovered that had paired heavy/light chain sequencing information and antigen/second antibody reactivity information from the AAB-seq experiment for SARS-COV-2 RBD antibodies (n = 3,712) are shown, with CLR(RBD_UMI) (x axis) and AAB-scores (y axis). Antibodies selected dispersedly for expression and validation (colorized dots) are colored by the LG(ELISA_AUC) scores from lowest (purple) to highest (red). (B) Neutralization assays were performed using pseudotyped viruses displaying the S protein from indicated SARS-CoV-2 WT. The values are means ± SD, n = 3. (C) Antibodies were tested for antibody-dependent cellular phagocytosis activity (ADCP) against SARS-CoV-2 RBD, compared to positive control <t>MM17T.</t> AUC of the trogocytosis score is shown, calculated from data in . Data are represented as means ± SDs, n=3. (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the C3b deposition score is shown, calculated from data in . Data are represented as means ± SDs, n=3.
Sars Cov 2 Spike Neutralizing Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sars cov 2 spike neutralizing antibody/product/Sino Biological
Average 93 stars, based on 1 article reviews
sars cov 2 spike neutralizing antibody - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier

Image Search Results


(A-B) A549:EV and A549:N cells were infected with WT (MOI 1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for confocal microscopy. (A-B) Maximum intensity projections of merged dsRNA ( A-B , red) staining with the N protein ( A , white) or Nsp6 ( B , white) in A549-EV and A549:N cells. Nuclei were Hoechst stained (blue). Note that DVG-B encoded GFP is depicted (green). Scale bar indicates 20μm. Insets depict magnified cell regions indicated by the white boxes of dsRNA and N protein co-staining ( A ) or dsRNA and Nsp6 co-staining ( B ). Scale bar indicates 5μm. Graphs indicate the Pearson’s correlation coefficient ( R) values for the co-localization of dsRNA and N ( C, above) or dsRNA and Nsp6 ( C, below ) in A549:EV and A549:N cells, respectively. Individual values are shown. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by one-way ANOVA with Turkey’s multiple comparisons test. Mean±SD. ( D ) A549:EV (left) and A549:N cells (right) were infected with WT (MOI 1) or DVG-B (MOI 50 to maximize the DVG-B infected cell number), respectively, and cells were collected 24-hours post-inoculation for fixation and processing for transmission electron microscopy. Scale bars indicate 2μm. Insets depict magnified cell regions indicated by the white boxes. Scale bars indicate 500nm. Black asterisks indicate single membrane vesicles, red asterisks indicate lysosome-like structures. ( E ) Due to significant more cell death in WT infected A549:EV and A549:N cells, we reduced MOI of WT virus to 0.1 for this experiment. A549:EV and A549:N cells were infected with WT (MOI 0.1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IFNB1, IL-29 and ISG54 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. Because the A549:N cell line expresses codon-optimized N, the qPCR primers specifically detect virus-derived N transcripts. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=2 biological repeats, mean±SD. ( F ) A549:EV and A549:N cells were inoculated as in ( E ). Immediately following 2-hours of inoculation, 10μg/mL of a neutralizing SARS-CoV-2 spike antibody or 10μg/mL of an IgG isotype control antibody were added to the infected cell cultures. 24-hours post inoculation cell supernatants were collected for virus titer determination by TCID 50 /mL quantification. Cells were collected for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IL-29 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=3 biological repeats, mean±SD.

Journal: bioRxiv

Article Title: SARS-CoV-2 Defective Viral Genomes from Distinct Genomic Regions Drive Divergent Interferon Responses

doi: 10.64898/2026.03.19.712870

Figure Lengend Snippet: (A-B) A549:EV and A549:N cells were infected with WT (MOI 1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for confocal microscopy. (A-B) Maximum intensity projections of merged dsRNA ( A-B , red) staining with the N protein ( A , white) or Nsp6 ( B , white) in A549-EV and A549:N cells. Nuclei were Hoechst stained (blue). Note that DVG-B encoded GFP is depicted (green). Scale bar indicates 20μm. Insets depict magnified cell regions indicated by the white boxes of dsRNA and N protein co-staining ( A ) or dsRNA and Nsp6 co-staining ( B ). Scale bar indicates 5μm. Graphs indicate the Pearson’s correlation coefficient ( R) values for the co-localization of dsRNA and N ( C, above) or dsRNA and Nsp6 ( C, below ) in A549:EV and A549:N cells, respectively. Individual values are shown. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by one-way ANOVA with Turkey’s multiple comparisons test. Mean±SD. ( D ) A549:EV (left) and A549:N cells (right) were infected with WT (MOI 1) or DVG-B (MOI 50 to maximize the DVG-B infected cell number), respectively, and cells were collected 24-hours post-inoculation for fixation and processing for transmission electron microscopy. Scale bars indicate 2μm. Insets depict magnified cell regions indicated by the white boxes. Scale bars indicate 500nm. Black asterisks indicate single membrane vesicles, red asterisks indicate lysosome-like structures. ( E ) Due to significant more cell death in WT infected A549:EV and A549:N cells, we reduced MOI of WT virus to 0.1 for this experiment. A549:EV and A549:N cells were infected with WT (MOI 0.1) or DVG-B (MOI 1), respectively, and cells were collected 24-hours post-inoculation for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IFNB1, IL-29 and ISG54 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. Because the A549:N cell line expresses codon-optimized N, the qPCR primers specifically detect virus-derived N transcripts. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=2 biological repeats, mean±SD. ( F ) A549:EV and A549:N cells were inoculated as in ( E ). Immediately following 2-hours of inoculation, 10μg/mL of a neutralizing SARS-CoV-2 spike antibody or 10μg/mL of an IgG isotype control antibody were added to the infected cell cultures. 24-hours post inoculation cell supernatants were collected for virus titer determination by TCID 50 /mL quantification. Cells were collected for RT-qPCR. Expression of host genes and viral genes/DVG-B were calculated relative to GAPDH. IL-29 relative copy number values were normalized to mock for A549:EV and A549:N, respectively. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by two-way ANOVA with Turkey’s multiple comparisons test. N=3 biological repeats, mean±SD.

Article Snippet: Post inoculation, cells were washed three times with PBS and cultured in 5% TCM with 10μg/mL of Spike neutralizing antibody (40592-R001, Sino Biological, obtained from BEI resources).

Techniques: Infection, Confocal Microscopy, Staining, Transmission Assay, Electron Microscopy, Membrane, Virus, Quantitative RT-PCR, Expressing, Derivative Assay, Control

Comparison of the SARS-CoV-2 polyclonal original-D614G, passage 60 and 71, and the recombinant P70rec viruses. ( a – c ) VeroCCL81 cells were infected at a multiplicity of infection of 0.001 with polyclonal passage (P) 4 (original-D614G virus), P60, or P71 virus stocks or the recombinant virus P70rec (n = 1 culture per virus), supernatant was collected at day 1–6 post-infection, and cultures were evaluated for ( a ) supernatant infectious titers (50% tissue culture infectious dose (TCID 50 )/mL) with dots representing geometric means of four technical replicates with standard deviations, ( b ) supernatant virus genome titers (RNA copies/mL), and ( c ) cytopathic effect evaluated by microscope inspection. ( d ) Original-D614G, P60, and P70rec viruses as specified above the graphs were produced in a fixed-bed bioreactor. Infectious titers were determined for the virus-containing medium collected upon each medium-replacement, bars represent geometric means of two to three technical replicates with standard deviations (left y-axis). The accumulated yield of TCID 50 units obtained from each culture was calculated based on the infectious titers and the volume of collected virus-containing medium (right y-axis). ( e ) 50% inhibitory dilution (ID 50 ) values against P60, P70rec, and P71 viruses for plasma from infection-naïve SARS-CoV-2 vaccinated (n = 6) or vaccination-naïve convalescent (n = 6) individuals. ( f ) 50% inhibitory concentration (IC 50 ) values for neutralization by monoclonal antibody bebtelovimab. ID 50 and IC 50 values were calculated based on curves shown in Supplementary Fig. . For ( e ) the three groups were compared in a Friedman test (line) with a post-hoc Dunn’s multiple comparison test (line with brackets). ** p < 0.005; ns, p > 0.05. LLOQ, lower limit of quantification. #, cytopathic effect not evaluated due to low numbers of cells because of SARS-CoV-2 induced cell death.

Journal: Scientific Reports

Article Title: An inactivated SARS-CoV-2 vaccine based on a Vero cell culture-adapted high-titer virus confers cross-protection in small animals

doi: 10.1038/s41598-024-67570-0

Figure Lengend Snippet: Comparison of the SARS-CoV-2 polyclonal original-D614G, passage 60 and 71, and the recombinant P70rec viruses. ( a – c ) VeroCCL81 cells were infected at a multiplicity of infection of 0.001 with polyclonal passage (P) 4 (original-D614G virus), P60, or P71 virus stocks or the recombinant virus P70rec (n = 1 culture per virus), supernatant was collected at day 1–6 post-infection, and cultures were evaluated for ( a ) supernatant infectious titers (50% tissue culture infectious dose (TCID 50 )/mL) with dots representing geometric means of four technical replicates with standard deviations, ( b ) supernatant virus genome titers (RNA copies/mL), and ( c ) cytopathic effect evaluated by microscope inspection. ( d ) Original-D614G, P60, and P70rec viruses as specified above the graphs were produced in a fixed-bed bioreactor. Infectious titers were determined for the virus-containing medium collected upon each medium-replacement, bars represent geometric means of two to three technical replicates with standard deviations (left y-axis). The accumulated yield of TCID 50 units obtained from each culture was calculated based on the infectious titers and the volume of collected virus-containing medium (right y-axis). ( e ) 50% inhibitory dilution (ID 50 ) values against P60, P70rec, and P71 viruses for plasma from infection-naïve SARS-CoV-2 vaccinated (n = 6) or vaccination-naïve convalescent (n = 6) individuals. ( f ) 50% inhibitory concentration (IC 50 ) values for neutralization by monoclonal antibody bebtelovimab. ID 50 and IC 50 values were calculated based on curves shown in Supplementary Fig. . For ( e ) the three groups were compared in a Friedman test (line) with a post-hoc Dunn’s multiple comparison test (line with brackets). ** p < 0.005; ns, p > 0.05. LLOQ, lower limit of quantification. #, cytopathic effect not evaluated due to low numbers of cells because of SARS-CoV-2 induced cell death.

Article Snippet: A SARS-CoV-2 neutralizing antibody (Sino Biological #40592-MM57; RRID: AB_2857935) served as a positive control for neutralization of the original-D614G virus, no positive control was included for neutralization of BA.1.

Techniques: Comparison, Recombinant, Infection, Virus, Microscopy, Produced, Concentration Assay, Neutralization

Neutralizing responses induced by SARS-CoV-2 mono- and bivalent inactivated vaccines in mice. ( a ) Mice were immunized three times with monovalent I-P60 (n = 4) or I-BA.1 (n = 4), bivalent I-P60/BA.1 (n = 4), or Ovalbumin (OVA) (n = 4) on day 0, 21, and 42, and serum was sampled on day 0, 14, 37, and 56. ( b ) Neutralization of homologous and heterologous viruses by serum was analyzed (left and right graphs for each group, respectively). In vaccines containing I-P60, original-D614G virus was used as the homologous virus in this assay. Dots represent values from individual animals, bars represent group means. < indicates that at least one value used to calculate the mean is below LLOQ; the LLOQ was determined as the first dilution tested in the assay, 1:12.5. Statistical analysis was only carried out between groups where all values were above the LLOQ. A Kruskal–Wallis test was done to compare more than two groups with the post-hoc Dunn’s multiple comparisons test (I-P60, I-P60/BA.1, and I-BA.1 serum from day 56 against BA.1). A Mann–Whitney test was used to compare two groups (at each timepoint, ID 50 values against each virus were compared for the inactivated vaccine groups). None of the tests showed significant differences. ID 50 values were calculated based on curves shown in Supplementary Fig. S3. ID 50 , 50% inhibitory dilution. LLOQ, lower limit of quantification.

Journal: Scientific Reports

Article Title: An inactivated SARS-CoV-2 vaccine based on a Vero cell culture-adapted high-titer virus confers cross-protection in small animals

doi: 10.1038/s41598-024-67570-0

Figure Lengend Snippet: Neutralizing responses induced by SARS-CoV-2 mono- and bivalent inactivated vaccines in mice. ( a ) Mice were immunized three times with monovalent I-P60 (n = 4) or I-BA.1 (n = 4), bivalent I-P60/BA.1 (n = 4), or Ovalbumin (OVA) (n = 4) on day 0, 21, and 42, and serum was sampled on day 0, 14, 37, and 56. ( b ) Neutralization of homologous and heterologous viruses by serum was analyzed (left and right graphs for each group, respectively). In vaccines containing I-P60, original-D614G virus was used as the homologous virus in this assay. Dots represent values from individual animals, bars represent group means. < indicates that at least one value used to calculate the mean is below LLOQ; the LLOQ was determined as the first dilution tested in the assay, 1:12.5. Statistical analysis was only carried out between groups where all values were above the LLOQ. A Kruskal–Wallis test was done to compare more than two groups with the post-hoc Dunn’s multiple comparisons test (I-P60, I-P60/BA.1, and I-BA.1 serum from day 56 against BA.1). A Mann–Whitney test was used to compare two groups (at each timepoint, ID 50 values against each virus were compared for the inactivated vaccine groups). None of the tests showed significant differences. ID 50 values were calculated based on curves shown in Supplementary Fig. S3. ID 50 , 50% inhibitory dilution. LLOQ, lower limit of quantification.

Article Snippet: A SARS-CoV-2 neutralizing antibody (Sino Biological #40592-MM57; RRID: AB_2857935) served as a positive control for neutralization of the original-D614G virus, no positive control was included for neutralization of BA.1.

Techniques: Vaccines, Neutralization, Virus, MANN-WHITNEY

Investigation of SARS-CoV-2 challenge dose in hamsters according to weight and infection. ( a ) Hamsters were challenged on day 0 with 2000 TCID 50 units of the original-D614G virus (n = 4) or the BA.1 virus (n = 4) or remained non-challenged (n = 4). Oropharynx swabs were obtained every day after challenge, lungs were collected on day 5 upon euthanasia. ( b ) Virus genome titers of oropharynx swabs. ( c ) Virus genome titers in lungs. For ( b ) and ( c ) symbols represent individual animals and lines represent group medians. < indicates that at least one value used to calculate the median is below LLOQ. ( d ) Body weight relative to the day of challenge, symbols represent group means with standard deviation, and symbols for the non-challenged group were nudged by − 0.1 on the x-axis. Statistical analysis was only carried out between groups where all values were above the LLOQ. For ( b ) and ( c ), a Mann–Whitney test was used to compare two groups. Only statistically significant differences are shown. For ( d ) a Kruskal–Wallis test (significance indicated above the symbols) was used at each timepoint; a post-hoc Dunn’s multiple comparison test showed that the significant difference was between the original-D614G and the non-challenged groups. * p < 0.05. LLOQ, lower limit of quantification.

Journal: Scientific Reports

Article Title: An inactivated SARS-CoV-2 vaccine based on a Vero cell culture-adapted high-titer virus confers cross-protection in small animals

doi: 10.1038/s41598-024-67570-0

Figure Lengend Snippet: Investigation of SARS-CoV-2 challenge dose in hamsters according to weight and infection. ( a ) Hamsters were challenged on day 0 with 2000 TCID 50 units of the original-D614G virus (n = 4) or the BA.1 virus (n = 4) or remained non-challenged (n = 4). Oropharynx swabs were obtained every day after challenge, lungs were collected on day 5 upon euthanasia. ( b ) Virus genome titers of oropharynx swabs. ( c ) Virus genome titers in lungs. For ( b ) and ( c ) symbols represent individual animals and lines represent group medians. < indicates that at least one value used to calculate the median is below LLOQ. ( d ) Body weight relative to the day of challenge, symbols represent group means with standard deviation, and symbols for the non-challenged group were nudged by − 0.1 on the x-axis. Statistical analysis was only carried out between groups where all values were above the LLOQ. For ( b ) and ( c ), a Mann–Whitney test was used to compare two groups. Only statistically significant differences are shown. For ( d ) a Kruskal–Wallis test (significance indicated above the symbols) was used at each timepoint; a post-hoc Dunn’s multiple comparison test showed that the significant difference was between the original-D614G and the non-challenged groups. * p < 0.05. LLOQ, lower limit of quantification.

Article Snippet: A SARS-CoV-2 neutralizing antibody (Sino Biological #40592-MM57; RRID: AB_2857935) served as a positive control for neutralization of the original-D614G virus, no positive control was included for neutralization of BA.1.

Techniques: Infection, Virus, Standard Deviation, MANN-WHITNEY, Comparison

Weight and tissue pathology in inactivated vaccine-immunized hamsters following SARS-CoV-2 challenge. ( a ) Hamsters were immunized twice with monovalent I-P60 (n = 8), bivalent I-P60/BA.1 (n = 8), or OVA (n = 12) on day 0 and 21, and plasma was sampled on day 0, 21, 42, and 49. On day 44, hamsters were challenged with 2000 TCID 50 units of the original-D614G virus or 6000 TCID 50 units of BA.1 (n = 4 per immunization group), or remained non-challenged (n = 4 for the OVA immunized group). ( b ) Body weight relative to the day of challenge, symbols represent group means with standard deviation. The non-challenged group is depicted in both graphs. ( c ) Lung inflammation scores determined as the percentage of lung tissue area affected by inflammation, dots represent animal means (determined from three tissue sections), bars represent group means also given as numbers above the bars. Mean scores for individual animals are given in Supplementary Table . ( d ) The number of animals per group with the indicated pathological changes in the lungs. Observations for individual animals are given in Supplementary Table . For ( b ) and ( c ) a Kruskal–Wallis test was used and significance is indicated above the symbols in ( b ) or as lines in ( c ) with a post-hoc Dunn’s multiple comparison test (line with brackets). Only statistically significant differences are shown. **** p < 0.0001; *** p < 0.0005; ** p < 0.005; * p < 0.05. OVA, Ovalbumin.

Journal: Scientific Reports

Article Title: An inactivated SARS-CoV-2 vaccine based on a Vero cell culture-adapted high-titer virus confers cross-protection in small animals

doi: 10.1038/s41598-024-67570-0

Figure Lengend Snippet: Weight and tissue pathology in inactivated vaccine-immunized hamsters following SARS-CoV-2 challenge. ( a ) Hamsters were immunized twice with monovalent I-P60 (n = 8), bivalent I-P60/BA.1 (n = 8), or OVA (n = 12) on day 0 and 21, and plasma was sampled on day 0, 21, 42, and 49. On day 44, hamsters were challenged with 2000 TCID 50 units of the original-D614G virus or 6000 TCID 50 units of BA.1 (n = 4 per immunization group), or remained non-challenged (n = 4 for the OVA immunized group). ( b ) Body weight relative to the day of challenge, symbols represent group means with standard deviation. The non-challenged group is depicted in both graphs. ( c ) Lung inflammation scores determined as the percentage of lung tissue area affected by inflammation, dots represent animal means (determined from three tissue sections), bars represent group means also given as numbers above the bars. Mean scores for individual animals are given in Supplementary Table . ( d ) The number of animals per group with the indicated pathological changes in the lungs. Observations for individual animals are given in Supplementary Table . For ( b ) and ( c ) a Kruskal–Wallis test was used and significance is indicated above the symbols in ( b ) or as lines in ( c ) with a post-hoc Dunn’s multiple comparison test (line with brackets). Only statistically significant differences are shown. **** p < 0.0001; *** p < 0.0005; ** p < 0.005; * p < 0.05. OVA, Ovalbumin.

Article Snippet: A SARS-CoV-2 neutralizing antibody (Sino Biological #40592-MM57; RRID: AB_2857935) served as a positive control for neutralization of the original-D614G virus, no positive control was included for neutralization of BA.1.

Techniques: Virus, Standard Deviation, Comparison

SARS-CoV-2 titers in upper and lower airways of inactivated vaccine-immunized hamsters following SARS-CoV-2 challenge. ( a – d ) Oropharynx swabs were obtained daily following challenge on day 44, according to the experiment outlined in Fig. , until euthanasia on day 49. ( a , c ) Virus genome titers following challenge with the original-D614G ( a ) or BA.1 ( c ) virus. The OVA immunized non-challenged group is included in both graphs for comparison. Curves represent group medians and dots represent individual values, n = 4 for each group except for the BA.1 challenged OVA immunized group on day 46 with n = 3 due to low sample recovery. ( b , d ) Infectious titers following challenge with the original-D614G ( b ) or BA.1 ( d ) virus; I-P60 and I-P60/BA.1 symbols in ( b ) and ( d ) were nudged by +/− 0.04, respectively, on the y-axis. The OVA immunized non-challenged group is included in both graphs for comparison. ( e ) Virus genome titers in lung tissue obtained on day 49, n = 4 for each group, LLOQ is based on measurements from the OVA immunized non-challenged group (mean plus three times the standard deviation). < indicates that at least one value used to calculate the median is below LLOQ, colored according to group. Statistical analysis was only carried out between groups where all values were above the LLOQ. A Kruskal–Wallis test was used (significance indicated above the symbols) with a post-hoc Dunn’s multiple comparison test (line with brackets). A Mann–Whitney test was used when only two groups were above LLOQ (not significant). Only statistically significant differences are shown. * p < 0.05. OVA, ovalbumin. TCID 50 , 50% tissue culture infectious dose. LLOQ, lower limit of quantification.

Journal: Scientific Reports

Article Title: An inactivated SARS-CoV-2 vaccine based on a Vero cell culture-adapted high-titer virus confers cross-protection in small animals

doi: 10.1038/s41598-024-67570-0

Figure Lengend Snippet: SARS-CoV-2 titers in upper and lower airways of inactivated vaccine-immunized hamsters following SARS-CoV-2 challenge. ( a – d ) Oropharynx swabs were obtained daily following challenge on day 44, according to the experiment outlined in Fig. , until euthanasia on day 49. ( a , c ) Virus genome titers following challenge with the original-D614G ( a ) or BA.1 ( c ) virus. The OVA immunized non-challenged group is included in both graphs for comparison. Curves represent group medians and dots represent individual values, n = 4 for each group except for the BA.1 challenged OVA immunized group on day 46 with n = 3 due to low sample recovery. ( b , d ) Infectious titers following challenge with the original-D614G ( b ) or BA.1 ( d ) virus; I-P60 and I-P60/BA.1 symbols in ( b ) and ( d ) were nudged by +/− 0.04, respectively, on the y-axis. The OVA immunized non-challenged group is included in both graphs for comparison. ( e ) Virus genome titers in lung tissue obtained on day 49, n = 4 for each group, LLOQ is based on measurements from the OVA immunized non-challenged group (mean plus three times the standard deviation). < indicates that at least one value used to calculate the median is below LLOQ, colored according to group. Statistical analysis was only carried out between groups where all values were above the LLOQ. A Kruskal–Wallis test was used (significance indicated above the symbols) with a post-hoc Dunn’s multiple comparison test (line with brackets). A Mann–Whitney test was used when only two groups were above LLOQ (not significant). Only statistically significant differences are shown. * p < 0.05. OVA, ovalbumin. TCID 50 , 50% tissue culture infectious dose. LLOQ, lower limit of quantification.

Article Snippet: A SARS-CoV-2 neutralizing antibody (Sino Biological #40592-MM57; RRID: AB_2857935) served as a positive control for neutralization of the original-D614G virus, no positive control was included for neutralization of BA.1.

Techniques: Virus, Comparison, Standard Deviation, MANN-WHITNEY

Neutralizing responses induced by SARS-CoV-2 mono- and bivalent inactivated vaccines in hamsters pre- and post-challenge. Plasma collected during the experiment outlined in Fig. at the indicated time points were analyzed for neutralization of the original-D614G and BA.1 viruses. ( a ) Plasma collected prior to challenge (I-P60 group n = 8, I-P60/BA.1 group n = 8, OVA group n = 12). ( b ) Plasma collected after challenge, n = 4 for each group. Dots represent values from individual animals, bars represent group means. < indicates that at least one value used to calculate the mean is below LLOQ; the LLOQ was determined as the first dilution tested in the assay, 1:25. Statistical analysis was only carried out between groups where all values were above the LLOQ. For ( a ), a Mann–Whitney test was done to compare groups (I-P60 against original-D614G vs. I-P60/BA.1 against original-D614G on day 21 and day 42). None of the tests showed significant differences. For ( b ), a Kruskal–Wallis test was used (line) with a post-hoc Dunn’s multiple comparison test (line with brackets). ID 50 values were calculated based on curves shown in Supplementary Fig. S5. * p < 0.05; ns, p > 0.05. ID 50 , 50% inhibitory dilution. LLOQ, lower limit of quantification. OVA, ovalbumin.

Journal: Scientific Reports

Article Title: An inactivated SARS-CoV-2 vaccine based on a Vero cell culture-adapted high-titer virus confers cross-protection in small animals

doi: 10.1038/s41598-024-67570-0

Figure Lengend Snippet: Neutralizing responses induced by SARS-CoV-2 mono- and bivalent inactivated vaccines in hamsters pre- and post-challenge. Plasma collected during the experiment outlined in Fig. at the indicated time points were analyzed for neutralization of the original-D614G and BA.1 viruses. ( a ) Plasma collected prior to challenge (I-P60 group n = 8, I-P60/BA.1 group n = 8, OVA group n = 12). ( b ) Plasma collected after challenge, n = 4 for each group. Dots represent values from individual animals, bars represent group means. < indicates that at least one value used to calculate the mean is below LLOQ; the LLOQ was determined as the first dilution tested in the assay, 1:25. Statistical analysis was only carried out between groups where all values were above the LLOQ. For ( a ), a Mann–Whitney test was done to compare groups (I-P60 against original-D614G vs. I-P60/BA.1 against original-D614G on day 21 and day 42). None of the tests showed significant differences. For ( b ), a Kruskal–Wallis test was used (line) with a post-hoc Dunn’s multiple comparison test (line with brackets). ID 50 values were calculated based on curves shown in Supplementary Fig. S5. * p < 0.05; ns, p > 0.05. ID 50 , 50% inhibitory dilution. LLOQ, lower limit of quantification. OVA, ovalbumin.

Article Snippet: A SARS-CoV-2 neutralizing antibody (Sino Biological #40592-MM57; RRID: AB_2857935) served as a positive control for neutralization of the original-D614G virus, no positive control was included for neutralization of BA.1.

Techniques: Vaccines, Neutralization, MANN-WHITNEY, Comparison

(A) All B cells (dots) recovered that had paired heavy/light chain sequencing information and antigen/second antibody reactivity information from the AAB-seq experiment for SARS-COV-2 RBD antibodies (n = 3,712) are shown, with CLR(RBD_UMI) (x axis) and AAB-scores (y axis). Antibodies selected dispersedly for expression and validation (colorized dots) are colored by the LG(ELISA_AUC) scores from lowest (purple) to highest (red). (B) Neutralization assays were performed using pseudotyped viruses displaying the S protein from indicated SARS-CoV-2 WT. The values are means ± SD, n = 3. (C) Antibodies were tested for antibody-dependent cellular phagocytosis activity (ADCP) against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the trogocytosis score is shown, calculated from data in . Data are represented as means ± SDs, n=3. (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the C3b deposition score is shown, calculated from data in . Data are represented as means ± SDs, n=3.

Journal: bioRxiv

Article Title: AAB-seq: An antigen-specific and affinity-readable high-throughput BCR sequencing method

doi: 10.1101/2024.08.13.607736

Figure Lengend Snippet: (A) All B cells (dots) recovered that had paired heavy/light chain sequencing information and antigen/second antibody reactivity information from the AAB-seq experiment for SARS-COV-2 RBD antibodies (n = 3,712) are shown, with CLR(RBD_UMI) (x axis) and AAB-scores (y axis). Antibodies selected dispersedly for expression and validation (colorized dots) are colored by the LG(ELISA_AUC) scores from lowest (purple) to highest (red). (B) Neutralization assays were performed using pseudotyped viruses displaying the S protein from indicated SARS-CoV-2 WT. The values are means ± SD, n = 3. (C) Antibodies were tested for antibody-dependent cellular phagocytosis activity (ADCP) against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the trogocytosis score is shown, calculated from data in . Data are represented as means ± SDs, n=3. (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the C3b deposition score is shown, calculated from data in . Data are represented as means ± SDs, n=3.

Article Snippet: MM17T (Sino biological, Cat:40592-MM17T) was used as a positive control.

Techniques: Sequencing, Expressing, Enzyme-linked Immunosorbent Assay, Neutralization, Activity Assay, Positive Control

(A) Neutralization assay data of pseudovirus neutralization(%) for SARS-CoV-2 WT. Data are represented as means ± SDs, n=3. (B) Antibodies were tested for antibody-dependent cellular phagocytosis activity against SARS-CoV-2 RBD, compared to positive control MM17T. Phagocytosis score (see Methods) is shown on the y-axis and antibody concentration is shown on the x-axis. Data are represented as means ± SDs, n=3. (C) Confirmation of phagocytosis by Confocal microscopy images. Nuclei are stained blue, beads red, and membrane in green with Concanavalin A(FITC-ConA). (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. Complement deposition score (see Methods) is shown on the y-axis and antibody concentration is shown on the x axis. Data are represented as means ± SDs, n=3. (E) Confocal microscopy images confirm the detection of complement deposition on fluorescent beads. Beads are stained red, and detection antibody in green with FITC-C3b antibody.

Journal: bioRxiv

Article Title: AAB-seq: An antigen-specific and affinity-readable high-throughput BCR sequencing method

doi: 10.1101/2024.08.13.607736

Figure Lengend Snippet: (A) Neutralization assay data of pseudovirus neutralization(%) for SARS-CoV-2 WT. Data are represented as means ± SDs, n=3. (B) Antibodies were tested for antibody-dependent cellular phagocytosis activity against SARS-CoV-2 RBD, compared to positive control MM17T. Phagocytosis score (see Methods) is shown on the y-axis and antibody concentration is shown on the x-axis. Data are represented as means ± SDs, n=3. (C) Confirmation of phagocytosis by Confocal microscopy images. Nuclei are stained blue, beads red, and membrane in green with Concanavalin A(FITC-ConA). (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. Complement deposition score (see Methods) is shown on the y-axis and antibody concentration is shown on the x axis. Data are represented as means ± SDs, n=3. (E) Confocal microscopy images confirm the detection of complement deposition on fluorescent beads. Beads are stained red, and detection antibody in green with FITC-C3b antibody.

Article Snippet: MM17T (Sino biological, Cat:40592-MM17T) was used as a positive control.

Techniques: Neutralization, Activity Assay, Positive Control, Concentration Assay, Confocal Microscopy, Staining, Membrane